AAV Rapid Quantitation Kit

AAV Rapid Quantitation Kit
  • Ultra-fast procedure (<2 hours)
  • Measures the viral nucleic acid content of unpurified AAV-2 or AAV-DJ supernatant or purified AAV of any serotype
  • Limit of detection: 10^9 GC/mL from unpurified AAV supernatant or 5 x 10^10 GC/mL for purified AAV


Frequently Asked Questions about this product

General FAQs about using AAV

General FAQs about Viral Gene Delivery

Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

QuickTiter™ AAV Quantitation Kit
Catalog Number
20 assays
Manual/Data Sheet Download
SDS Download
QuickTiter™ AAV Quantitation Kit, Trial Size
Catalog Number
4 assays
Manual/Data Sheet Download
SDS Download
Product Details

Traditionally, adeno-associated virus (AAV) particles have been quantified by DNA dot blot or similar procedures. These methods can be time-consuming and suffer from a high degree of inter-assay variability.

Our QuickTiter™ AAV Quantitation Kit uses a proprietary technology to quantify the viral nucleic acid content of AAV preps. The kit can be used with unpurified supernatant of AAV-2 or AAV-DJ, or with purified AAV from any serotype.

Assay Principle for the QuickTiter™ AAV Quantitation Kit.

Standard Curve Generated with the QuickTiter™ AAV Quantitation Kit.The QuickTiter™ AAV-2 DNA Standard was diluted as described in the Assay Protocol. Fluorescence measurement was performed on a SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff.

Recent Product Citations
  1. Lebeau, P.F. et al. (2020). The loss-of-function PCSK9Q152H variant increases ER chaperones GRP78 and GRP94 and protects against liver injury. J Clin Invest. doi: 10.1172/JCI128650.
  2. Kawaguchi, Y. et al. (2020). Endoplasmic reticulum chaperone BiP/GRP78 knockdown leads to autophagy and cell death of arginine vasopressin neurons in mice. Sci Rep. 10(1):19730. doi: 10.1038/s41598-020-76839-z.
  3. Rai, R. et al. (2020). Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott - Aldrich Syndrome. Nat Commun. 11(1):4034. doi: 10.1038/s41467-020-17626-2.
  4. Zeng, J. et al (2020). The Zika Virus Capsid Disrupts Corticogenesis by Suppressing Dicer Activity and miRNA Biogenesis. Cell Stem Cell. S1934-5909(20)30350-7. doi: 10.1016/j.stem.2020.07.012.
  5. Tang, Y. et al. (2020). Social touch promotes interfemale communication via activation of parvocellular oxytocin neurons. Nat Neurosci. doi: 10.1038/s41593-020-0674-y.
  6. Zhu, J. et al. (2020). Preparation of a Bacteriophage T4-based Prokaryotic-eukaryotic Hybrid Viral Vector for Delivery of Large Cargos of Genes and Proteins into Human Cells. Bio-protocol. 10(07): e3573. doi: 10.21769/BioProtoc.3573.
  7. Wu, Z. et al. (2020). Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 11(1):1105. doi: 10.1038/s41467-020-14855-3.
  8. Zhang, H. et al. (2020). Vitamin D receptor targets hepatocyte nuclear factor 4α and mediates protective effects of vitamin D in nonalcoholic fatty liver disease. J Biol Chem. pii: jbc.RA119.011487. doi: 10.1074/jbc.RA119.011487.
  9. Xu, Y. et al. (2020). Diabetic nephropathy execrates epithelial-to-mesenchymal transition (EMT) via miR-2467-3p/Twist1 pathway. Biomed Pharmacother. 125:109920. doi: 10.1016/j.biopha.2020.109920.
  10. Oser, M.G. et al. (2019). The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesis. Genes Dev. doi: 10.1101/gad.328336.119.
  11. Niranjan, N. et al. (2019). Sarcolipin overexpression impairs myogenic differentiation in Duchenne muscular dystrophy. Am J Physiol Cell Physiol. doi: 10.1152/ajpcell.00146.2019.
  12. Ferretti, V. et al. (2019). Oxytocin Signaling in the Central Amygdala Modulates Emotion Discrimination in Mice. Curr Biol. pii: S0960-9822(19)30499-3. doi: 10.1016/j.cub.2019.04.070.
  13. Hasan, M.T. et al. (2019). A Fear Memory Engram and Its Plasticity in the Hypothalamic Oxytocin System. Neuron. pii: S0896-6273(19)30386-1. doi: 10.1016/j.neuron.2019.04.029.
  14. Lee, S. et al. (2019). Anti-EpCAM-conjugated adeno-associated virus serotype 2 for systemic delivery of EGFR shRNA: Its retargeting and antitumor effects on OVCAR3 ovarian cancer in vivo. Acta Biomater. pii: S1742-7061(19)30287-9. doi: 10.1016/j.actbio.2019.04.044.
  15. Nakamura, M. et al. (2019). Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy. Cell Metab. pii: S1550-4131(19)30005-1. doi: 10.1016/j.cmet.2019.01.005.
  16. Tseng, S.J. et al. (2018). Targeting Tumor Microenvironment by Bioreduction-Activated Nanoparticles for Light-Triggered Virotherapy. ACS Nano. 12(10):9894-9902. doi: 10.1021/acsnano.8b02813.
  17. Ikegami, R. et al. (2018). Gamma-Aminobutyric Acid Signaling in Brown Adipose Tissue Promotes Systemic Metabolic Derangement in Obesity. Cell Rep. 24(11):2827-2837.e5. doi: 10.1016/j.celrep.2018.08.024.
  18. Menon, R. et al. (2018). Oxytocin Signaling in the Lateral Septum Prevents Social Fear during Lactation. Curr Biol. 28(7):1066-1078.e6. doi: 10.1016/j.cub.2018.02.044.
  19. Wen, L. et al. (2018). Transient High Pressure in Pancreatic Ducts Promotes Inflammation and Alters Tight Junctions via Calcineurin Signaling in Mice. Gastroenterology. 155(4):1250-1263.e5. doi: 10.1053/j.gastro.2018.06.036.
  20. McGrady NR, et al. (2017). Upregulation of the endothelin A (ETA) receptor and its association with neurodegeneration in a rodent model of glaucoma. BMC Neurosci. 18(1):27. doi: 10.1186/s12868-017-0346-3.
  21. Wang, P., et al. (2017). Tau interactome mapping based identification of Otub1 as Tau deubiquitinase involved in accumulation of pathological Tau forms in vitro and in vivo. Acta Neuropathol. doi:10.1007/s00401-016-1663-9.
  22. Tseng, S.J. et al. (2016). Remote control of light-triggered virotherapy. ACS Nano 10:10339-10346.
  23. Eliava, M. et al. (2016). A new population of parvocellular oxytocin neurons controlling magnocellular neuron activity and inflammatory pain processing. Neuron 89:1291-1304.
  24. Madroñal, N. et al. (2016). Rapid erasure of hippocampal memory following inhibition of dentate gyrus granule cells. Nat Commun. doi:10.1038/ncomms10923.
  25. Park, H. Y. et al. (2016). Gadd45β ameliorates L-DOPA-induced dyskinesia in a Parkinson's disease mouse model. Neurobiol Dis. 89:169-179.
  26. Tran, L. & Keele, N. B. (2016). CaMKIIα knockdown decreases anxiety in the open field and low serotonin-induced upregulation of GluA1 in the basolateral amygdala. Behav Brain Res. 303:152-159.
  27. Chen, W. et al. (2016). The silencing of cathepsin K used in gene therapy for periodontal disease reveals the role of cathepsin K in chronic infection and inflammation. J Periodontal Res.doi:10.1111/jre.12345.
  28. Orabi, A. I. et al. (2015). Dynamic imaging of pancreatic NF-κB activation in live mice using AAV infusion and bioluminescence. J Biol Chem. doi:10.1074/jbc.M115.647933.
  29. Oh, S. M. et al. (2015). Combined Nurr1 and Foxa2 roles in the therapy of Parkinson's disease. EMBO Mol Med. doi: 10.15252/emmm.201404610. 
  30. Stankowska, D. L. et al. (2015). Neuroprotective effects of transcription factor brn3b in an ocular hypertension rat model of glaucoma. Invest Ophthalmol Vis Sci.  doi: 10.1167/iovs.14-15008.