AAV Rapid Quantitation Kit

AAV Rapid Quantitation Kit
  • Ultra-fast procedure (<2 hours)
  • Measures the viral nucleic acid content of unpurified AAV-2 or AAV-DJ supernatant or purified AAV of any serotype
  • Limit of detection: 10^9 GC/mL from unpurified AAV supernatant or 5 x 10^10 GC/mL for purified AAV


Frequently Asked Questions about this product

General FAQs about using AAV

General FAQs about Viral Gene Delivery

Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

QuickTiter™ AAV Quantitation Kit
Catalog Number
20 assays
Manual/Data Sheet Download
SDS Download
Product Details

Traditionally, adeno-associated virus (AAV) particles have been quantified by DNA dot blot or similar procedures. These methods can be time-consuming and suffer from a high degree of inter-assay variability.

Our QuickTiter™ AAV Quantitation Kit uses a proprietary technology to quantify the viral nucleic acid content of AAV preps. The kit can be used with unpurified supernatant of AAV-2 or AAV-DJ, or with purified AAV from any serotype.

Assay Principle for the QuickTiter™ AAV Quantitation Kit.

Standard Curve Generated with the QuickTiter™ AAV Quantitation Kit.The QuickTiter™ AAV-2 DNA Standard was diluted as described in the Assay Protocol. Fluorescence measurement was performed on a SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff.

Recent Product Citations
  1. Iwasaki, M. et al. (2023). An analgesic pathway from parvocellular oxytocin neurons to the periaqueductal gray in rats. Nat Commun. 14(1):1066. doi: 10.1038/s41467-023-36641-7.
  2. Yoshizawa, T. et al. (2022). SIRT7 suppresses energy expenditure and thermogenesis by regulating brown adipose tissue functions in mice. Nat Commun. 13(1):7439. doi: 10.1038/s41467-022-35219-z.
  3. Furuuchi, R. et al. (2022). Endothelial SIRT-1 has a critical role for the maintenance of capillarization in brown adipose tissue. iScience. doi: 10.1016/j.isci.2022.105424.
  4. Kennedy, A. et al. (2022). Differences in CD80 and CD86 transendocytosis reveal CD86 as a key target for CTLA-4 immune regulation. Nat Immunol. 23(9):1365-1378. doi: 10.1038/s41590-022-01289-w.
  5. Hsieh, C.C. et al. (2022). Macrophage Distribution Affected by Virus-Encoded Granulocyte Macrophage Colony Stimulating Factor Combined with Lactate Oxidase. ACS Omega. doi: 10.1021/acsomega.2c03213.
  6. Hayashi, Y. et al. (2022). Coagulation factors promote brown adipose tissue dysfunction and abnormal systemic metabolism in obesity. iScience. doi: 10.1016/j.isci.2022.104547.
  7. Yoshida, Y. et al. (2022). Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue. iScience. doi: 10.1016/j.isci.2022.104268.
  8. Xu, M. et al. (2022). The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes. Nat Commun. 13(1):1052. doi: 10.1038/s41467-022-28641-w.
  9. Kashihara, T. et al. (2022). YAP mediates compensatory cardiac hypertrophy through aerobic glycolysis in response to pressure overload. J Clin Invest. doi: 10.1172/JCI150595.
  10. Paiva, L. et al. (2021). Identification of peripheral oxytocin-expressing cells using systemically applied cell-type specific adeno-associated viral vector. J Neuroendocrinol. doi: 10.1111/jne.12970.
  11. Wahis, J. et al. (2021). Astrocytes mediate the effect of oxytocin in the central amygdala on neuronal activity and affective states in rodents. Nat Neurosci. doi: 10.1038/s41593-021-00800-0.
  12. Francisco, J. et al. (2021). AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis. Sci Rep. 11(1):10553. doi: 10.1038/s41598-021-89989-5.
  13. Kwon, O.C. et al. (2021). SGK1 inhibition in glia ameliorates pathologies and symptoms in Parkinson disease animal models. EMBO Mol Med. doi: 10.15252/emmm.202013076.
  14. Lebeau, P.F. et al. (2020). The loss-of-function PCSK9Q152H variant increases ER chaperones GRP78 and GRP94 and protects against liver injury. J Clin Invest. doi: 10.1172/JCI128650.
  15. Kawaguchi, Y. et al. (2020). Endoplasmic reticulum chaperone BiP/GRP78 knockdown leads to autophagy and cell death of arginine vasopressin neurons in mice. Sci Rep. 10(1):19730. doi: 10.1038/s41598-020-76839-z.
  16. Rai, R. et al. (2020). Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott - Aldrich Syndrome. Nat Commun. 11(1):4034. doi: 10.1038/s41467-020-17626-2.
  17. Zeng, J. et al (2020). The Zika Virus Capsid Disrupts Corticogenesis by Suppressing Dicer Activity and miRNA Biogenesis. Cell Stem Cell. S1934-5909(20)30350-7. doi: 10.1016/j.stem.2020.07.012.
  18. Tang, Y. et al. (2020). Social touch promotes interfemale communication via activation of parvocellular oxytocin neurons. Nat Neurosci. doi: 10.1038/s41593-020-0674-y.
  19. Zhu, J. et al. (2020). Preparation of a Bacteriophage T4-based Prokaryotic-eukaryotic Hybrid Viral Vector for Delivery of Large Cargos of Genes and Proteins into Human Cells. Bio-protocol. 10(07): e3573. doi: 10.21769/BioProtoc.3573.
  20. Wu, Z. et al. (2020). Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 11(1):1105. doi: 10.1038/s41467-020-14855-3.
  21. Zhang, H. et al. (2020). Vitamin D receptor targets hepatocyte nuclear factor 4α and mediates protective effects of vitamin D in nonalcoholic fatty liver disease. J Biol Chem. pii: jbc.RA119.011487. doi: 10.1074/jbc.RA119.011487.
  22. Xu, Y. et al. (2020). Diabetic nephropathy execrates epithelial-to-mesenchymal transition (EMT) via miR-2467-3p/Twist1 pathway. Biomed Pharmacother. 125:109920. doi: 10.1016/j.biopha.2020.109920.
  23. Oser, M.G. et al. (2019). The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesis. Genes Dev. doi: 10.1101/gad.328336.119.
  24. Niranjan, N. et al. (2019). Sarcolipin overexpression impairs myogenic differentiation in Duchenne muscular dystrophy. Am J Physiol Cell Physiol. doi: 10.1152/ajpcell.00146.2019.
  25. Ferretti, V. et al. (2019). Oxytocin Signaling in the Central Amygdala Modulates Emotion Discrimination in Mice. Curr Biol. pii: S0960-9822(19)30499-3. doi: 10.1016/j.cub.2019.04.070.
  26. Hasan, M.T. et al. (2019). A Fear Memory Engram and Its Plasticity in the Hypothalamic Oxytocin System. Neuron. pii: S0896-6273(19)30386-1. doi: 10.1016/j.neuron.2019.04.029.
  27. Lee, S. et al. (2019). Anti-EpCAM-conjugated adeno-associated virus serotype 2 for systemic delivery of EGFR shRNA: Its retargeting and antitumor effects on OVCAR3 ovarian cancer in vivo. Acta Biomater. pii: S1742-7061(19)30287-9. doi: 10.1016/j.actbio.2019.04.044.
  28. Nakamura, M. et al. (2019). Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy. Cell Metab. pii: S1550-4131(19)30005-1. doi: 10.1016/j.cmet.2019.01.005.
  29. Tseng, S.J. et al. (2018). Targeting Tumor Microenvironment by Bioreduction-Activated Nanoparticles for Light-Triggered Virotherapy. ACS Nano. 12(10):9894-9902. doi: 10.1021/acsnano.8b02813.
  30. Ikegami, R. et al. (2018). Gamma-Aminobutyric Acid Signaling in Brown Adipose Tissue Promotes Systemic Metabolic Derangement in Obesity. Cell Rep. 24(11):2827-2837.e5. doi: 10.1016/j.celrep.2018.08.024.