Cell Based Exogenous Antioxidant Assay

Cell Based Exogenous Antioxidant Assay
  • Determine the efficacy of an exogenous antioxidant compound within a cellular environment
  • Each kit provides sufficient reagents to perform up to 192 assays, including standard curve and unknown samples
Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

OxiSelect™ Cellular Antioxidant Activity Assay Kit (Green Fluorescence)
Catalog Number
192 assays
Manual/Data Sheet Download
SDS Download
Product Details

The OxiSelect™ Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

Recent Product Citations
  1. Donadio, G. et al. (2023). Characterization of Health Beneficial Components in Discarded Leaves of Three Escarole (Cichorium endivia L.) Cultivar and Study of Their Antioxidant and Anti-Inflammatory Activities. Antioxidants. 12(7):1402. doi: 10.3390/antiox12071402.
  2. Kusznierewicz, B. et al. (2021). Comparative evaluation of different methods for determining phytochemicals and antioxidant activity in products containing betalains – Verification of beetroot samples. Food Chem. doi: 10.1016/j.foodchem.2021.130132.
  3. Xiong, Y. et al. (2021). In vitro and cellular antioxidant activities of 3-deoxyanthocyanidin colourants. Food Biosci. doi: 10.1016/j.fbio.2021.101171.
  4. Xiong, Y. et al. (2021). Cellular antioxidant activities of phenolic extracts from five sorghum grain genotypes. Food Biosci. doi: 10.1016/j.fbio.2021.101068.
  5. Baranowska, M. et al. (2021). Interactions between polyphenolic antioxidants quercetin and naringenin dictate the distinctive redox-related chemical and biological behaviour of their mixtures. Sci Rep. 11(1):12282. doi: 10.1038/s41598-021-89314-0.
  6. Huguet-Casquero, A. et al. (2020). Encapsulation of Oleuropein in Nanostructured Lipid Carriers: Biocompatibility and Antioxidant Efficacy in Lung Epithelial Cells. Pharmaceutics. 12(5). pii: E429. doi: 10.3390/pharmaceutics12050429.
  7. Baranowska, M. et al. (2020). Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract. Free Radic Biol Med. pii: S0891-5849(20)30504-9. doi: 10.1016/j.freeradbiomed.2020.04.022.
  8. Koss-Mikołajczyk, I. et al. (2019). The Relationship between Phytochemical Composition and Biological Activities of Differently Pigmented Varieties of Berry Fruits; Comparison between Embedded in Food Matrix and Isolated Anthocyanins. Foods. 8(12). pii: E646. doi: 10.3390/foods8120646.
  9. Koss-Mikołajczyk, I. et al. (2019). Phytochemical composition and biological activities of differently pigmented cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis) varieties. J Sci Food Agric. doi: 10.1002/jsfa.9811.
  10. Todorovic, V. et al. (2019). Regulation of Cellular Redox Homeostasis by (–)-Epicatechin and Cocoa Extracts—A Pilot Study. Proceedings. 11(1):6. doi: 10.3390/proceedings2019011006.
  11. Koziara, Z. et al. (2019). Comparison of Redox Properties of Flavonoid Aglycones and Corresponding Glycosides and Their Mixtures in the Cellular Model. Proceedings. 11(1):25. doi: 10.3390/proceedings2019011025.
  12. Nolte, J. et al. (2019). Glucansucrase catalyzed synthesis and functional characterization of nordihydroguaiaretic acid glucosides. Enzyme Microb Technol. 120:69-76. doi: 10.1016/j.enzmictec.2018.10.002.
  13. Kellett, M.E. et al. (2018). Modification of the cellular antioxidant activity (CAA) assay to study phenolic antioxidants in a Caco-2 cell line. Food Chem. 244:359-363. doi: 10.1016/j.foodchem.2017.10.035.
  14. Pagano, I. et al. (2016). Chemical profile and cellular antioxidant activity of artichoke by-products. Food Funct. doi:10.1039/C6FO01443G.
  15. Chiang, M. et al. (2016).  Chemical-and cell-based antioxidant capacity of methanolic extracts of three commonly edible plants from Zingiberaceae family. Free Radical Antioxid. 7:57-62.
  16. Verbanac, D. et al. (2016). Synthesis and evaluation of antibacterial and antioxidant activity of novel 2-phenyl-quinoline analogs derivatized at position 4 with aromatically substituted 4 H-1, 2, 4-triazoles. J Enzyme Inhib Med Chem. doi:10.1080/14756366.2016.1190714.
  17. Yamani, H. & Abbenante, G. (2015). The OxiSelect Cellular Antioxidant Assay (CAA) on the FLUOstar Omega. BMG Labtech Application Notes.