Our OxiSelect™ UV-Induced DNA Damage ELISA Kit measures the formation of cyclobutane pyrimidine dimers (CPD) in DNA isolated from cells or tissues. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate. The sample or standard are then probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody.
DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The CPD levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for CPD.