CPD ELISA for Isolated DNA

CPD ELISA for Isolated DNA
  • Measure CPD structures of DNA lesions induced by UV light
  • Detect structures in isolated DNA using standard ELISA plate format

 

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OxiSelect™ UV-Induced DNA Damage ELISA Kit (CPD Quantitation)
Catalog Number
STA-322
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$540.00
OxiSelect™ UV-Induced DNA Damage ELISA Kit (CPD Quantitation)
Catalog Number
STA-322-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$2,325.00
Product Details

Our OxiSelect™ UV-Induced DNA Damage ELISA Kit measures the formation of cyclobutane pyrimidine dimers (CPD) in DNA isolated from cells or tissues. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate.  The sample or standard are then probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. 

DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The CPD levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for CPD.

Recent Product Citations
  1. Holzscheck, N. et al. (2020). Concomitant DNA methylation and transcriptome signatures define epidermal responses to acute solar UV radiation. Sci Rep. 10(1):12918. doi: 10.1038/s41598-020-69683-8.
  2. Shapira, Y. et al. (2019). Pre-germination plasma treatment of seeds does not alter cotyledon DNA structure, nor phenotype and phenology of tomato and pepper plants. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2019.09.034.
  3. Chazarin, B. et al. (2019). Limited Oxidative Stress Favors Resistance to Skeletal Muscle Atrophy in Hibernating Brown Bears (Ursus Arctos). Antioxidants (Basel). 8(9). pii: E334. doi: 10.3390/antiox8090334.
  4. Innes, S.N. et al. (2019). Elevated air humidity increases UV mediated leaf and DNA damage in pea (Pisum sativum) due to reduced flavonoid content and antioxidant power. Photochem Photobiol Sci. 18(2):387-399. doi: 10.1039/c8pp00401c.
  5. Zhao, H. et al. (2018). Modulation of DNA Repair Systems in Blind Cavefish during Evolution in Constant Darkness. Curr Biol. 28(20):3229-3243.e4. doi: 10.1016/j.cub.2018.08.039.
  6. Cui, H. et al. (2016). The Stress-responsive Gene ATF3 mediates dichotomous UV responses by regulating the Tip60 and p53 proteins. J Biol Chem. 291:10847-10857.
  7. Rimann, M. et al. (2015). Standardized 3D bioprinting of soft tissue models with human primary cells. J Lab Autom. doi:10.1177/2211068214567146.
  8. Donninger, H. et al. (2015). The RASSF1A tumor suppressor regulates XPA-mediated DNA repair. Mol Cell Biol. 35:277-287.
  9. Zirkin, S. et al. (2013). The PIM-2 Kinase Is an Essential Component of the Ultraviolet Damage Response That Acts Upstream to E2F-1 and ATM. J. Biol. Chem. 288:21770-21789 (#STA-322).
  10. Burgess, H.M. et al. (2011). Nuclear Relocalisation of Cytoplasmic poly(A)-binding Proteins PABP1 and PABP4 in Response to UV Irradiation Reveals mRNA-dependent Export of Metazoan PABPs. J. Cell Sci. 124:3344-3355 (#STA-322).