CPD Staining Kit

CPD Staining Kit
  • Measure CPD structures of DNA lesions induced by UV light
  • Detect structures by immunofluorescence staining under a fluorescence microscope
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OxiSelect™ Cellular UV-Induced DNA Damage Staining Kit (CPD)
Catalog Number
STA-327
Size
96 assays
Detection
Fluorescence Microscopy
Manual/Data Sheet Download
SDS Download
Price
$550.00
Product Details

Our OxiSelect™ Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

DNA Damage Induced by UV Light in Hela Cells. HeLa cells were seeded at 20K/well overnight, then exposed to light under a germicidal lamp for 30 minutes. Immunofluorescence staining of CPD damage was determined as described in the Assay Protocol for #STA-327.

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