DAG Assay Kit

DAG Assay Kit
  • Suitable for use with cell lysates
  • Detection sensitivity of 4 µM DAG
  • DAG standard include

NOTE: Each sample replicate requires 2 assays for proper data calculation.

Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

DAG (Diacylglycerol) Assay Kit
Catalog Number
MET-5028
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$425.00
Product Details

Diacylglycerol (DAG) is a glyceride consisting of two fatty acid chains linked by a central glycerol backbone. It is a precursor to lipids such as triglycerides and phospholipids and functions as a second messenger signaling lipid. It is produced through hydrolysis of PIP2 by phospholipase C, which initiates intracellular Ca2+ release and PKC activation. Activated PKC enzymes interact with many different substrates, affecting cellular physiology.

Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.

Recent Product Citations
  1. Chiang, Y.P. et al. (2021). Sphingomyelin synthase related protein is a mammalian phosphatidylethanolamine phospholipase C. Biochim Biophys Acta Mol Cell Biol Lipids. 1866(11):159017. doi: 10.1016/j.bbalip.2021.159017.
  2. Brekk, O.R. et al. (2020). Cell type-specific lipid storage changes in Parkinson's disease patient brains are recapitulated by experimental glycolipid disturbance. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.2003021117.
  3. Koundouros, N. et al. (2020). Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids. Cell. doi: 10.1016/j.cell.2020.05.053.
  4. Day, P. et al. (2019). Inhibitors of diacylglycerol metabolism suppress CCR2 receptor signalling in human monocytes. Br J Pharmacol. doi: 10.1111/bph.14695.
  5. Badi, R.M. et al. (2019). Resveratrol protects against hepatic insulin resistance in a rat's model of non-alcoholic fatty liver disease by down-regulation of GPAT-1 and DGAT2 expression and inhibition of PKC membranous translocation. Clin Exp Pharmacol Physiol. doi: 10.1111/1440-1681.13074.
  6. Garcia, D. et al. (2019). Genetic Liver-Specific AMPK Activation Protects against Diet-Induced Obesity and NAFLD. Cell Rep. 26(1):192-208.e6. doi: 10.1016/j.celrep.2018.12.036.
  7. Fanning, S. et al. (2019). Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell. 73(5):1001-1014.e8. doi: 10.1016/j.molcel.2018.11.028.
  8. Kang, R. et al. (2018). Lipid Peroxidation Drives Gasdermin D-Mediated Pyroptosis in Lethal Polymicrobial Sepsis. Cell Host Microbe. 24(1):97-108.e4. doi: 10.1016/j.chom.2018.05.009.
  9. Tanaka, N., et al. (2017). Growth arrest and DNA damage-inducible 45α protects against nonalcoholic steatohepatitis induced by methionine- and choline-deficient diet. Biochim Biophys Acta. pii: S0925-4439(17)30299-5. doi: 10.1016/j.bbadis.2017.08.017.