DAG Assay Kit

  • Suitable for use with cell lysates
  • Detection sensitivity of 4 µM DAG
  • DAG standard include

NOTE: Each sample replicate requires 2 assays for proper data calculation.

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DAG (Diacylglycerol) Assay Kit
Catalog Number
MET-5028
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$395.00
Product Details

Diacylglycerol (DAG) is a glyceride consisting of two fatty acid chains linked by a central glycerol backbone. It is a precursor to lipids such as triglycerides and phospholipids and functions as a second messenger signaling lipid. It is produced through hydrolysis of PIP2 by phospholipase C, which initiates intracellular Ca2+ release and PKC activation. Activated PKC enzymes interact with many different substrates, affecting cellular physiology.

Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.