TBARS (Lipid Peroxidation) Assay

TBARS Assay Kit
  • Quick screening tool for oxidative stress
  • User friendly protocol uses smaller reaction volumes in a 96-well format
  • Does not require glass tubes or marbles


Frequently Asked Questions about this product

General FAQs about Oxidative Stress

Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

OxiSelect™ TBARS Assay Kit (MDA Quantitation)
Catalog Number
200 assays
Colorimetric / Fluorometric
Manual/Data Sheet Download
SDS Download
OxiSelect™ TBARS Assay Kit (MDA Quantitation)
Catalog Number
5 x 200 assays
Colorimetric / Fluorometric
Manual/Data Sheet Download
SDS Download
Product Details

The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidationMDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured.

Our OxiSelect™ TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required.

Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

MDA-TBA Adduct

OxiSelect™ TBARS Assay Kit Standard Curve with Colorimetric Detection. The MDA standard curve can also be generated using fluorometric detection.

Recent Product Citations
  1. Deng, Z. et al. (2023). Efficacy of soy protein concentrate replacing animal protein supplements in mucosa-associated microbiota, intestinal health, and growth performance of nursery pigs. Animal Nutrition. doi: 10.1016/j.aninu.2023.06.007.
  2. Deng, Z. et al. (2023). Comparative effects of soy protein concentrate, enzyme-treated soybean meal, and fermented soybean meal replacing animal protein supplements in feeds on growth performance and intestinal health of nursery pigs. J Anim Sci Biotechnol. 14(1):89. doi: 10.1186/s40104-023-00888-3.
  3. Abdallah, M. S. et al. (2023). Therapeutic Management, Clinicopathological, Molecular and Cost Studies on Sarcoptes scabiei Infestation in Rabbit. J. Adv. Vet. 13(3):333-338.
  4. Won, S.Y. et al. (2023). Effect of individual or combination of dietary betaine and glycine on productive performance, stress response, liver health, and intestinal barrier function in broiler chickens raised under heat stress conditions. Poult Sci. 102(7):102771. doi: 10.1016/j.psj.2023.102771.
  5. Hemraj, D. A. et al. (2023). Acidification and hypoxia drive physiological trade-offs in oysters and partial loss of nutrient cycling capacity in oyster holobiont. Front Ecol Evol. doi: 10.3389/fevo.2023.1083315.
  6. Mayer, W. et al. (2023). Biomolecules of Fermented Tropical Fruits and Fermenting Microbes as Regulators of Human Hair Loss, Hair Quality, and Scalp Microbiota. Biomolecules. 13(4):699. doi: 10.3390/biom13040699.
  7. Leanza, G. et al. (2023). Oxidative Stress in Postmenopausal Women with or without Obesity. Cells. 12(8):1137. doi: 10.3390/cells12081137.
  8. Nikolic, A. et al. (2023). Chronic stress targets mitochondrial respiratory efficiency in the skeletal muscle of C57BL/6 mice. Cell Mol Life Sci. 80(4):108. doi: 10.1007/s00018-023-04761-4.
  9. Šķesters, A. et al. (2023). Selenium Status and Oxidative Stress in SARS-CoV-2 Patients. Medicina (Kaunas). 59(3):527. doi: 10.3390/medicina59030527.
  10. Baik, K. Y. et al. (2023). Synergistic Effect of Hydrogen Peroxide and Cold Atmospheric Pressure Plasma-Jet for Microbial Disinfection. Applied Sciences. 13(5):3324. doi: 10.3390/app13053324.
  11. Peris-Martínez, C. et al. (2023). Antioxidant and Anti-Inflammatory Effects of Oral Supplementation with a Highly-Concentrated Docosahexaenoic Acid (DHA) Triglyceride in Patients with Keratoconus: A Randomized Controlled Preliminary Study. Nutrients. 15(5):1300. doi: 10.3390/nu15051300.
  12. Dos Anjos, C. et al. (2023). New Insights into the Bacterial Targets of Antimicrobial Blue Light. Microbiol Spectr. 11(2):e0283322. doi: 10.1128/spectrum.02833-22.
  13. Jack, B.U. et al. (2023). Cyclopia intermedia (Honeybush) Induces Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor Alpha Expression in Obese Diabetic Female db/db Mice. Int J Mol Sci. 24(4):3868. doi: 10.3390/ijms24043868.
  14. Hashida, M. et al. (2023).  α-Tocopherol Transfer Protein-Null Mice with Very Low α-Tocopherol Status Do Not Have an Enhanced Lipopolysaccharide-Induced Acute Inflammatory Response. Curr Dev Nutr. doi: 10.1016/j.cdnut.2022.100017.
  15. Kosutova, P. et al. (2022). Time-Dependent Oxidative Alterations in Plasma and Lung Tissue after Meconium Aspiration in a Rabbit Model. Antioxidants (Basel). 12(1):37. doi: 10.3390/antiox12010037.
  16. Lee, J.I. et al. (2023). Transcriptomic and phenotypic changes of Cronobacter sakazakii ATCC 29544 grown under desiccation stress. LWT. doi: 10.1016/j.lwt.2022.114279.
  17. Jeon, H.J. et al. (2023). Developmental toxicity of chlorpyrifos-methyl and its primary metabolite, 3,5,6-trichloro-2-pyridinol to early life stages of zebrafish (Danio rerio). Ecotoxicol Environ Saf. doi: 10.1016/j.ecoenv.2022.114352.
  18. Tanawattanasuntorn, T. et al. (2022). Trans-(±)-Kusunokinin Binding to AKR1B1 Inhibits Oxidative Stress and Proteins Involved in Migration in Aggressive Breast Cancer. Antioxidants (Basel). 11(12):2347. doi: 10.3390/antiox11122347.
  19. Ryu, J.H. et al. (2022). Fermented and Aged Ginseng Sprouts (Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis. Antioxidants. 11(10):2052. doi: 10.3390/antiox11102052.
  20. Lee, J.H. et al. (2022). Evaluation of tryptophan biomass as an alternative to conventional crystalline tryptophan in broiler diets. J Appl Poult Res. doi: 10.1016/j.japr.2022.100302.
  21. Kim, Y.S. et al. (2022). Fermented Laminaria japonica improves working memory and antioxidant defense mechanism in healthy adults: a randomized, double-blind, and placebo-controlled clinical study. Fish Aquat Sci. 25(8):450-461. doi: 10.47853/FAS.2022.e41.
  22. Rajab, B.S. et al. (2022). Antioxidative and Anti-Inflammatory Protective Effects of β-Caryophyllene against Amikacin-Induced Nephrotoxicity in Rat by Regulating the Nrf2/AMPK/AKT and NF-κB/TGF-β/KIM-1 Molecular Pathways. Oxid Med Cell Longev. doi: 10.1155/2022/4212331.
  23. Kushwah, A.S. et al. (2022). Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model. Evid Based Complement Alternat Med. doi: 10.1155/2022/6874281.
  24. Deng, Z. et al. (2022). Soy protein concentrate replacing animal protein supplements and its impacts on intestinal immune status, intestinal oxidative stress status, nutrient digestibility, mucosa-associated microbiota, and growth performance of nursery pigs. J Anim Sci. doi: 10.1093/jas/skac255.
  25. Vasavda, C. et al. (2022). Identification of the NRF2 transcriptional network as a therapeutic target for trigeminal neuropathic pain. Sci Adv. 8(31):eabo5633. doi: 10.1126/sciadv.abo5633.
  26. Navarro, J.A. et al. (2022). Endocrine and Metabolic Impact of Oral Ingestion of a Carob-Pod-Derived Natural-Syrup-Containing D-Pinitol: Potential Use as a Novel Sweetener in Diabetes. Pharmaceutics. 14(8):1594. doi: 10.3390/pharmaceutics14081594.
  27. Całyniuk, Z. et al. (2022). The effect of the application of diets with varied proportions of arginine and lysine on biochemical and antioxidant status in Turkeys. Ann. Anim. Sci. 22(3):1041-1055. doi: 10.2478/aoas-2021-0081.
  28. Dugbartey, G.J. et al. (2022). Alpha-lipoic acid treatment improves adverse cardiac remodelling in the diabetic heart - The role of cardiac hydrogen sulfide-synthesizing enzymes. Biochem Pharmacol. doi: 10.1016/j.bcp.2022.115179.
  29. Tolouee, M. et al. (2022). Cooling of Cells and Organs Confers Extensive DNA Strand Breaks Through Oxidative Stress and ATP Depletion. Cell Transplant. doi: 10.1177/09636897221108705.
  30. Tascón, J. et al. (2022). Early Diagnosis of Kidney Damage Associated with Tobacco Use: Preventive Application. J Pers Med. 12(7):1032. doi: 10.3390/jpm12071032.