TBARS (Lipid Peroxidation) Assay

TBARS Assay Kit
  • Quick screening tool for oxidative stress
  • User friendly protocol uses smaller reaction volumes in a 96-well format
  • Does not require glass tubes or marbles


Frequently Asked Questions about this product

General FAQs about Oxidative Stress

Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

OxiSelect™ TBARS Assay Kit (MDA Quantitation)
Catalog Number
200 assays
Colorimetric / Fluorometric
Manual/Data Sheet Download
SDS Download
OxiSelect™ TBARS Assay Kit (MDA Quantitation)
Catalog Number
5 x 200 assays
Colorimetric / Fluorometric
SDS Download
Product Details

The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidationMDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured.

Our OxiSelect™ TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required.

Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

MDA-TBA Adduct

OxiSelect™ TBARS Assay Kit Standard Curve with Colorimetric Detection. The MDA standard curve can also be generated using fluorometric detection.

Recent Product Citations
  1. Dos Anjos, C. et al. (2023). New Insights into the Bacterial Targets of Antimicrobial Blue Light. Microbiol Spectr. 11(2):e0283322. doi: 10.1128/spectrum.02833-22.
  2. Jack, B.U. et al. (2023). Cyclopia intermedia (Honeybush) Induces Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor Alpha Expression in Obese Diabetic Female db/db Mice. Int J Mol Sci. 24(4):3868. doi: 10.3390/ijms24043868.
  3. Hashida, M. et al. (2023).  α-Tocopherol Transfer Protein-Null Mice with Very Low α-Tocopherol Status Do Not Have an Enhanced Lipopolysaccharide-Induced Acute Inflammatory Response. Curr Dev Nutr. doi: 10.1016/j.cdnut.2022.100017.
  4. Kosutova, P. et al. (2022). Time-Dependent Oxidative Alterations in Plasma and Lung Tissue after Meconium Aspiration in a Rabbit Model. Antioxidants (Basel). 12(1):37. doi: 10.3390/antiox12010037.
  5. Lee, J.I. et al. (2023). Transcriptomic and phenotypic changes of Cronobacter sakazakii ATCC 29544 grown under desiccation stress. LWT. doi: 10.1016/j.lwt.2022.114279.
  6. Jeon, H.J. et al. (2023). Developmental toxicity of chlorpyrifos-methyl and its primary metabolite, 3,5,6-trichloro-2-pyridinol to early life stages of zebrafish (Danio rerio). Ecotoxicol Environ Saf. doi: 10.1016/j.ecoenv.2022.114352.
  7. Tanawattanasuntorn, T. et al. (2022). Trans-(±)-Kusunokinin Binding to AKR1B1 Inhibits Oxidative Stress and Proteins Involved in Migration in Aggressive Breast Cancer. Antioxidants (Basel). 11(12):2347. doi: 10.3390/antiox11122347.
  8. Ryu, J.H. et al. (2022). Fermented and Aged Ginseng Sprouts (Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis. Antioxidants. 11(10):2052. doi: 10.3390/antiox11102052.
  9. Lee, J.H. et al. (2022). Evaluation of tryptophan biomass as an alternative to conventional crystalline tryptophan in broiler diets. J Appl Poult Res. doi: 10.1016/j.japr.2022.100302.
  10. Kim, Y.S. et al. (2022). Fermented Laminaria japonica improves working memory and antioxidant defense mechanism in healthy adults: a randomized, double-blind, and placebo-controlled clinical study. Fish Aquat Sci. 25(8):450-461. doi: 10.47853/FAS.2022.e41.
  11. Rajab, B.S. et al. (2022). Antioxidative and Anti-Inflammatory Protective Effects of β-Caryophyllene against Amikacin-Induced Nephrotoxicity in Rat by Regulating the Nrf2/AMPK/AKT and NF-κB/TGF-β/KIM-1 Molecular Pathways. Oxid Med Cell Longev. doi: 10.1155/2022/4212331.
  12. Kushwah, A.S. et al. (2022). Cardioprotective Activity of Cassia fistula L. Bark Extract in Isoproterenol-Induced Myocardial Infarction Rat Model. Evid Based Complement Alternat Med. doi: 10.1155/2022/6874281.
  13. Deng, Z. et al. (2022). Soy protein concentrate replacing animal protein supplements and its impacts on intestinal immune status, intestinal oxidative stress status, nutrient digestibility, mucosa-associated microbiota, and growth performance of nursery pigs. J Anim Sci. doi: 10.1093/jas/skac255.
  14. Vasavda, C. et al. (2022). Identification of the NRF2 transcriptional network as a therapeutic target for trigeminal neuropathic pain. Sci Adv. 8(31):eabo5633. doi: 10.1126/sciadv.abo5633.
  15. Navarro, J.A. et al. (2022). Endocrine and Metabolic Impact of Oral Ingestion of a Carob-Pod-Derived Natural-Syrup-Containing D-Pinitol: Potential Use as a Novel Sweetener in Diabetes. Pharmaceutics. 14(8):1594. doi: 10.3390/pharmaceutics14081594.
  16. Całyniuk, Z. et al. (2022). The effect of the application of diets with varied proportions of arginine and lysine on biochemical and antioxidant status in Turkeys. Ann. Anim. Sci. 22(3):1041-1055. doi: 10.2478/aoas-2021-0081.
  17. Dugbartey, G.J. et al. (2022). Alpha-lipoic acid treatment improves adverse cardiac remodelling in the diabetic heart - The role of cardiac hydrogen sulfide-synthesizing enzymes. Biochem Pharmacol. doi: 10.1016/j.bcp.2022.115179.
  18. Tolouee, M. et al. (2022). Cooling of Cells and Organs Confers Extensive DNA Strand Breaks Through Oxidative Stress and ATP Depletion. Cell Transplant. doi: 10.1177/09636897221108705.
  19. Tascón, J. et al. (2022). Early Diagnosis of Kidney Damage Associated with Tobacco Use: Preventive Application. J Pers Med. 12(7):1032. doi: 10.3390/jpm12071032.
  20. Sidharta, B.R.A. et al. (2022). Single or Divided Administration of Cisplatin Can Induce Inflammation and Oxidative Stress in Male Sprague-Dawley Rats. Indones Biomed J. 14(2): 164-71. doi: 10.18585/inabj.v14i2.1745.
  21. Jiang, M. et al. (2022). Association of DNA methylation in circulating CD4+T cells with short-term PM2.5 pollution waves: A quasi-experimental study of healthy young adults. Ecotoxicol Environ Saf. doi: 10.1016/j.ecoenv.2022.113634.
  22. Sedky, A.A. et al. (2022). Effects of tamoxifen alone and in combination with risperidone on hyperlocomotion, hippocampal structure and bone in ketamine-induced model of psychosis in rats. Egypt J Neurol Psychiatry Neurosurg. doi: 10.1186/s41983-022-00470-0.
  23. Bokhary, T. et al. (2022). Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation, and apoptosis in rats. J Integr Med. doi: 10.1016/j.joim.2022.05.001.
  24. Shao, J. et al. (2022). Maternal omega-3 fatty acid supplementation against prenatal lead exposure induced cognitive impairment in offspring mice. J Toxicol Sci. 47(5):183-192. doi: 10.2131/jts.47.183.
  25. Emam, K.K. et al. (2022). Assessment of Wheat Germ Oil Role in the Prevention of Induced Breast Cancer in Rats. ACS Omega. 7(16):13942-13952. doi: 10.1021/acsomega.2c00434.
  26. Holanda, D.M. & Kim, S.W. (2022). Impacts of weaning weights and mycotoxin challenges on jejunal mucosa-associated microbiota, intestinal and systemic health, and growth performance of nursery pigs. J Anim Sci Biotechnol. 13(1):43. doi: 10.1186/s40104-022-00691-6.
  27. Moita, V.H.C. et al. (2022). Functional roles of xylanase enhancing intestinal health and growth performance of nursery pigs by reducing the digesta viscosity and modulating the mucosa-associated microbiota in the jejunum. J Anim Sci. doi: 10.1093/jas/skac116.
  28. Palipoch, S., et al. (2022). Aqueous Thunbergia laurifolia leaf extract alleviates paraquat-induced lung injury in rats by inhibiting oxidative stress and inflammation. BMC Complement Med Ther. 22(1):83. doi: 10.1186/s12906-022-03567-4.
  29. Rodríguez-Pérez, M.D. et al. (2022). Neuroprotective Effect of 3',4'-Dihydroxyphenylglycol in Type-1-like Diabetic Rats-Influence of the Hydroxytyrosol/3',4'-dihydroxyphenylglycol Ratio. Nutrients. 14(6):1146. doi: 10.3390/nu14061146.
  30. Kim, Y.B. et al. (2022). Incorporation of Dietary Methyl Sulfonyl Methane into the Egg Albumens of Laying Hens. Antioxidants (Basel). 11(3):517. doi: 10.3390/antiox11030517.