Total Glutathione Assay

Total Glutathione Assay
  • Measures total glutathione (oxidized and reduced)
  • Sensitive detection as low as 8 nM
  • Suitable for use with serum, plasma, saliva, urine, tissue extracts, and mammalian or plant cell lysates


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OxiSelect™ Total Glutathione (GSSG/GSH) Assay Kit
Catalog Number
100 assays
Manual/Data Sheet Download
SDS Download
Product Details

The OxiSelect™ Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG).  Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH.  Subsequently, the  chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm.  The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve.  The rate of chromophore production is proportional to the concentration of glutathione within the sample.  The rate can be determined from the absorbance change over time.  Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.

GSSG Standard Curve. OD 405nm versus incubation time as a function of GSSG concentration.

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