In Vitro Nitric Oxide Assays

In Vitro Nitric Oxide Assays
  • Measure NO in various samples by NO2-/NO3-determination
  • Suitable for serum, plasma, urine, saliva, lysates, and media
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OxiSelect™ In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit
Catalog Number
STA-802
Size
100 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$340.00
OxiSelect™ In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit
Catalog Number
STA-802-5
Size
5 x 100 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$1,515.00
OxiSelect™ In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit
Catalog Number
STA-801
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$385.00
OxiSelect™ In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit
Catalog Number
STA-801-5
Size
5 x 100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$1,595.00
Product Details

Cell Biolabs’ OxiSelect™ Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination.  First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme.  Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm).  Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples.  The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume). 

Recent Product Citations
  1. Ishikawa, K. et al. (2020). Non-thermal plasma-activated lactate solution kills U251SP glioblastoma cells in an innate reductive manner with altered metabolism. Arch Biochem Biophys. doi: 10.1016/j.abb.2020.108414 (#STA-802).
  2. Kang, J.Y. et al. (2020). Therapeutic potential of miR-21 regulation by human peripheral blood derived-small extracellular vesicles in myocardial infarction. Clin Sci (Lond). 134(8):985-999. doi: 10.1042/CS20191077 (#STA-802).
  3. Jain, S. et al. (2020). Quantitative proteomics reveal an altered pattern of protein expression in saliva of hypobaric hypoxia-induced rat model. J Proteins Proteom. doi: 10.1007/s42485-020-00037-8 (#STA-801).
  4. Jain, S. et al. (2019). Saliva panel of protein candidates: A comprehensive study for assessing high altitude acclimatization. Nitric Oxide. 95:1-11. doi: 10.1016/j.niox.2019.11.007 (#STA-801).
  5. Park, J. et al. (2019). Non-thermal atmospheric pressure plasma induces epigenetic modifications that activate the expression of various cytokines and growth factors in human mesoderm-derived stem cells. Free Radic Biol Med. 148:108-122. doi: 10.1016/j.freeradbiomed.2019.12.035 (#STA-802).
  6. Signorello, M.G. et al. (2019). The Ca2+/calmodulin kinase/AMP-activated protein kinase pathway regulates the lectin phaseolus vulgaris agglutinin induced NO production in human platelets. Integr Mol Med. doi: 10.15761/IMM.1000369 (#STA-802).
  7. Tanzilli, G. et al. (2019). Glutathione infusion before primary percutaneous coronary intervention: a randomised controlled pilot study. BMJ Open. 9(8):e025884. doi: 10.1136/bmjopen-2018-025884 (#STA-802).
  8. Nier, A. et al. (2019). Short-Term Isocaloric Intake of a Fructose- but not Glucose-Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight Healthy Subjects. Mol Nutr Food Res. 63(6):e1800868. doi: 10.1002/mnfr.201800868 (#STA-802).
  9. Mohd Heikal, M.Y. et al. (2019). Stichopus chloronotus aqueous extract as a chondroprotective agent for human chondrocytes isolated from osteoarthitis articular cartilage in vitro. Cytotechnology. doi: 10.1007/s10616-019-00298-2 (#STA-802).
  10. Abdrabouh, A.E. (2019). Liver disorders related to exposure to gasoline fumes in male rats and role of fenugreek seed supplementation. Environ Sci Pollut Res Int. 26(9):8949-8957. doi: 10.1007/s11356-019-04307-x (#STA-802).
  11. Kurake, N. et al. (2019). Non-thermal plasma-activated medium modified metabolomic profiles in the glycolysis of U251SP glioblastoma. Arch Biochem Biophys. 662:83-92. doi: 10.1016/j.abb.2018.12.001 (#STA-802).
  12. Takahashi, Y. et al. (2018). Cytotoxicity of cancer HeLa cells sensitivity to normal MCF10A cells in cultivations with cell culture medium treated by microwave-excited atmospheric pressure plasmas. Journal of Physics D: Applied Physics. 51(11):115401. doi:10.1088/1361-6463/aaab09 (#STA-802).
  13. Beltrán-Barrientos, L.M. et al. (2018). Mechanistic Pathways Underlying the Antihypertensive Effect of Fermented Milk with Lactococcus lactis NRRL B-50571 in Spontaneously Hypertensive Rats. Nutrients. 10(3). pii: E262. doi: 10.3390/nu10030262 (#STA-802).
  14. Brubaker, T. R.. et al. (2017). Dynamic analysis of reactive oxygen nitrogen species in plasma-activated culture medium by UV absorption spectroscopy. Journal of Applied Physics. 122(21), 213301. doi:10.1063/1.4999256 (#STA-802).
  15. Li, M. et al. (2017). Targeting reactive nitrogen species suppresses hereditary pancreatic cancer. PNAS USA114(27):7106-7111 (#STA-802).
  16. Vincent, A. et al. (2016). Herring and chicken/pork meals lead to differences in plasma levels of TCA intermediates and arginine metabolites in overweight and obese men and women. Mol. Nut. Food Res. doi:10.1002/mnfr.201600400 (#STA-802).
  17. Son, D.J. et al. (2016). Novel synthetic (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) phenol inhibits arthritis by targeting signal transducer and activator of transcription 3. Sci. Rep. 6:36852 (#STA-802).
  18. Wang, Z. et al. (2015). Dual-microstructured porous, anisotropic film for biomimicking of endothelial basement membrane. ACS Appl Mater Interfaces. doi:10.1021/acsami.5b02464 (#STA-802).