Total Antioxidant Capacity (TAC) Assay

  • Measures total antioxidant capacity based on reduction of copper(II) to copper(I)
  • Suitable for use with plasma, serum, urine, cell lysates, tissue homogenates and food extracts
  • Works with a wide variety of antioxidants


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OxiSelect™ Total Antioxidant Capacity (TAC) Assay Kit
Catalog Number
200 assays
Manual/Data Sheet Download
SDS Download
OxiSelect™ Total Antioxidant Capacity (TAC) Assay Kit, Trial Size
Catalog Number
20 assays
Manual/Data Sheet Download
SDS Download
Product Details

The OxiSelect™ Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm.

TAC Assay Principle.

TAC Assay Standard Curve.

TAC Assay Measurement with Various Antioxidants.

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  1. Feng, J. et al. (2016). Inhibitor of nicotinamide phosphoribosyltransferase sensitizes glioblastoma cells to temozolomide via activating ROS/JNK signaling pathway. BioMed Res. Int. doi:10.1155/2016-1450843.
  2. Braud, L. et al. (2016). Antioxidant properties of tea blunt ROS-dependent lipogenesis: beneficial effect on hepatic steatosis in a high fat-high sucrose diet NAFLD obese rat model. J. Nut. Biochem. 40:95-104.
  3. Ciesla, M. et al. (2016). Heme oxygenase-1 controls an HDAC4-miR 206 pathway of oxidative stress in rhabdomyosarcoma. Cancer Res. 76:5707-5718.
  4. Harisa, G. I. et al. (2016). Simvastatin-loaded nanostructured lipid carriers attenuate the atherogenic risk of erythrocytes in hyperlipidemic rats. Eur J Pharm Sci. 96:62-71.
  5. Kawano, T. et al. (2016). Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging. Sci Rep. doi:10.1038/srep32604.
  6. Zbynovska, K. et al. (2016). Antioxidant status of rabbits after treatment with epicatechin and patulin. Biologia. 71:835-842.
  7. Lao, T. et al. (2016). Hhip haploinsufficiency sensitizes mice to age-related emphysema. Proc Natl Acad Sci U S A. doi:10.1073/pnas.1602342113.
  8. Nyembe, D. W. et al. (2016). Effects of ingested multi-walled carbon nanotubes in Poecilia reticulata: Localization and physiological responses. J Environ Anal Toxicol. 6:2161-0525.
  9. Harisa, G. I. et al. (2016). Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte’s arginase activation. Saudi Pharm J. doi:10.1016/j.jsps.2016.05.007.
  10. Wang, M. et al. (2016). Does total antioxidant capacity modify adverse cardiac responses associated with ambient ultrafine, accumulation mode, and fine particles in patients undergoing cardiac rehabilitation?. Environ Res. 149:15-22.
  11. Patel, S. et al. (2016). Vitamin A or E and a catechin synergize as vaccine adjuvant to enhance immune responses in mice by induction of early IL-15 but not IL-1β responses. Immunology. doi:10.1111/imm.12614.
  12. Simide, R. et al. (2016). Assessment of the accuracy of physiological blood indicators for the evaluation of stress, health status and welfare in Siberian sturgeon (Acipenser baerii) subject to chronic heat stress and dietary supplementation. Int Aquatic Res. doi:10.1007/s40071-016-0128-z.
  13. El-Deeb, M. M. K. et al. (2016). The role of serum trace elements and oxidative stress in egyptian breast cancer patients. Adv Breast Cancer Res. 5:37-47.
  14. Milewski, K. et al. (2016). Carnosine reduces oxidative stress and reverses attenuation of righting and postural reflexes in rats with thioacetamide-induced liver failure. Neurochem Res. doi:10.1007/s11064-015-1821-9.
  15. Kim, M. et al. (2015). Comparison of viability and antioxidant capacity between canine adipose-derived mesenchymal stem cells and heme oxygenase-1-overexpressed cells after freeze-thawing. J Vet Med Sci. doi:10.1292/jvms.15-0361.
  16. Wilcoxen, T. E. et al. (2015). Effects of bird-feeding activities on the health of wild birds. Conserv Physiol. doi:10.1093/conphys/cov058.
  17. Dong, W. et al. (2015). Enhancement of thiamin content in Arabidopsis thaliana by metabolic engineering. Plant Cell Physiol. 56:2285-96.
  18. Stensland, I. et al. (2015). A comparison of diets supplemented with a feed additive containing organic acids, cinnamaldehyde and a permeabilizing complex, or zinc oxide, on post-weaning diarrhoea, selected bacterial populations, blood measures and performance in weaned pigs experimentally infected with enterotoxigenic E. coli. Animals. 5:1147-1168.
  19. Pandupuspitasari, N. S. et al. (2015). Effects of diludine on the production, oxidative status, and biochemical parameters in transition cows. J Environ Agric Sci. 6:3-9.
  20. Strahl, J. et al. (2015). Biochemical responses to ocean acidification contrast between tropical corals with high and low abundances at volcanic carbon dioxide seeps. ICES J Mar Sci.  doi:10.1093/icesjms/fsv194.
  21. Agar, O. T. et al. (2015). Comparative studies on phenolic composition, antioxidant, wound healing and cytotoxic activities of selected Achillea L. species growing in turkey. Molecules. 20:17976-18000.
  22. Wu, J. et al. (2015). Cyclic and constant hyperoxia cause inflammation, apoptosis and cell death in human umbilical vein endothelial cells. Acta Anaesthesiol Scand. doi:10.1111/aas.12646.
  23. Alagbonsi, I. A. et al. (2015). Melatonin and vitamin C exacerbate Cannabis sativa-induced testicular damage when administered separately but ameliorate it when combined in rats. J Basic Clin Physiol Pharmacol. doi:10.1515/jbcpp-2015-0061.
  24. Hwang, H. J. et al. (2015). Production of Liquiritigenin with Cell-based Biotransformation and Its Anti-Aging Activity. Korean Soc Biotechnol Bioeng J. 30:166-174.
  25. Ni, R. et al. (2015). Mitochondrial calpain-1 disrupts ATP synthase and induces superoxide generation in type-1 diabetic hearts: a novel mechanism contributing to diabetic cardiomyopathy. Diabetes. doi:10.2337/db15-0963.
  26. Liu, M. et al. (2015). Shen-Kang protects 5/6 nephrectomized rats against renal injury by reducing oxidative stress through the MAPK signaling pathways. Int J Mol Med. 36:975-984.
  27. Scholten, S. D. et al. (2015). Effects of vitamin D and quercetin, alone and in combination, on cardiorespiratory fitness and muscle function in physically active male adults. Open Access J Sports Med.6:229-239.
  28. Pérez, E. et al. (2015). Improved antitumor effect of paclitaxel administered in vivo as pH and glutathione-sensitive nanohydrogels. Int J Pharm. 492:10-19.
  29. Antonopoulos, A. S. et al. (2015). Adiponectin as a link between type 2 diabetes and vascular NADPH oxidase activity in the human arterial wall: The regulatory role of perivascular adipose tissue. Diabetes. 64:2207-2219.
  30. Turkmenoglu, F. P. et al. (2015). Characterization of volatile compounds of eleven achillea species from turkey and biological activities of essential oil and methanol extract of A. hamzaoglui arabacı & budak. Molecules. 20:11432-11458.
  31. Sun, M. & Johnson, M. A. (2015). Measurement of total antioxidant capacity in sub-μL blood samples using craft paper-based analytical devices. RSC Adv. 5:55633-55639.
  32. Mikail, M. A. et al. (2015). Baccaurea angulata fruit inhibits lipid peroxidation and induces the increase in antioxidant enzyme activities. Eur J Nutr. doi:10.1007/s00394-015-0961-7.
  33. Ruiz, L. M. et al. (2015). Quercetin affects erythropoiesis and heart mitochondrial function in mice. Oxid Med Cell Longev. doi:10.1155/2015/836301
  34. Park, S. Y. et al. (2015). Study on the health benefits of brown algae (Sargassum muticum) in volunteers.  J Food Nutr Res. 3:126-130.
  35. Beretta, G. L. et al. (2015). Unravelling “off-target” effects of redox-active polymers and polymer multilayered capsules in prostate cancer cells. Nanoscale. doi: 10.1039/C4NR07240E.
  36. Youn, P. et al. (2015). Cytoprotection against beta-amyloid (Aβ) peptide-mediated oxidative damage and autophagy by Keap1 RNAi in human glioma U87mg cells. Neurosci Res. doi:10.1016/j.neures.2014.12.015.
  37. Sashindran, R., et al. (2015). Evaluation of neuroprotective effect of Quercetin and coenzyme Q10 in ethanol induced neurotoxicity in mice.Int J Appl Biol Pharm. 6:67-71.
  38. Sutkowy, P. et al. (2014). Physical exercise combined with whole-body cryotherapy in evaluating the level of lipid peroxidation products and other oxidant stress indicators in kayakers. Oxid Med Cell Longev. doi:10.1155/2014/402631.
  39. Mercado, N. et al. (2014). Activation of transcription factor Nrf2 signalling by the sphingosine kinase inhibitor SKI-II is mediated by the formation of Keap1 dimers. PLoS One. 9: e88168.
  40. Chien, J. W. et al. (2014). Urinary 8-hydroxy-2'-deoxyguanosine (8-oxodG) level can predict acute renal damage in young children with urinary tract infection.Biomarkers. 19:326-331.
  41. Narayanan, S. et al. (2014). Impact of high night-time and high daytime temperature stress on winter wheat. Journal of Agronomy and Crop Science. doi: 10.1111/jac.12101.
  42. Abudabos, A. M. & Al-Mufarrej, S. I. (2014). Effects of organic acid supplementation on antioxidant capacity and immune responses of broilers challenged orally with Salmonella enterica subsp. enterica Typhimurium. S Afr J Anim Sci. 44:360-370.
  43. Girgih, A. T. et al. (2014). A novel hemp seed meal protein hydrolysate reduces oxidative stress factors in spontaneously hypertensive rats. Nutrients. 6:5652-5666.
  44. Mesárošová, M. et al. (2014). The role of reactive oxygen species in the genotoxicity of surface-modified magnetite nanoparticles. Toxicol Lett. 226:303-313.
  45. Bello, M. H. et al. (2014). Endogenous ergothioneine is required for wild type levels of conidiogenesis and conidial survival but does not protect against 254nm UV-induced mutagenesis or kill. Fungal Genet Biol. 73:120-127.
  46. Moosa, Z. M. et al. (2014). The effects of prenatal methylmercury exposure on trace element and antioxidant levels in rats following 6-hydroxydopamine-induced neuronal insult. Metab Brain Dis. 29:459-469.
  47. Tran, H. et al. (2014).  Effects of Spray-Dried Porcine Plasma on Growth Performance, Immune Response, Total Antioxidant Capacity, and Gut Morphology of Nursery Pigs. J Anim Sci. 92:4494-4504.
  48. Ravikumar, P. et al. (2014).  α-Klotho Protects Against Oxidative Damage in Pulmonary Epithelia. Am J Physiol Lung Cell Mol Physiol. 307:L566-575.
  49. MacDonald, G. et al. (2014). Memo Is a Copper-Dependent Redox Protein with an Essential Role in Migration and Metastasis. Sci Signal. 7:ra56.
  50. Stark, M. et al. (2013). Differential Effects of Docosahexaenoic Acid on Preterm and Term Placental Pro-Oxidant/Antioxidant Balance. Reproduction. 146: 243-251.
  51. Bakalova, R. et al. (2013). Tissue Redox Activity as a Hallmark of Carcinogenesis: From Early to Terminal Stages of Cancer. Clin. Cancer Res. 19:2503-2517.
  52. Wang, Y. et al. (2013). Therapeutic Effect of MG-132 on Diabetic Cardiomyopathy is Associated with its Suppression of Proteasomal Activities: Roles of Nrf2 and NF-B. Am J Physiol Heart Circ Physiol. 304:H567-H578.
  53. Chen, L. et al. (2012). OPA1 Mutation and Late-Onset Cardiomyopathy: Mitochondrial Dysfunction and mtDNA Instability. JAHA. 1: e003012.
  54. Dittrich,R. et al. (2012). Influence of Maternal Smoking during Pregnancy on Oxidant Status in Amniotic Fluid. In Vivo. 26: 813-818.