Total Antioxidant Capacity (TAC) Assay

Total Antioxidant Capacity (TAC) Assay
  • Measures total antioxidant capacity based on reduction of copper(II) to copper(I)
  • Suitable for use with plasma, serum, urine, cell lysates, tissue homogenates and food extracts
  • Works with a wide variety of antioxidants


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OxiSelect™ Total Antioxidant Capacity (TAC) Assay Kit
Catalog Number
200 assays
Manual/Data Sheet Download
SDS Download
Product Details

The OxiSelect™ Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm.

TAC Assay Principle.

TAC Assay Standard Curve.

TAC Assay Measurement with Various Antioxidants.

Recent Product Citations
  1. Lingens, J.B. et al. (2021). Evaluation of Methionine Sources in Protein Reduced Diets for Turkeys in the Late Finishing Period Regarding Performance, Footpad Health and Liver Health. Agriculture. 11(9):901. doi: 10.3390/agriculture11090901.
  2. Ahmed-Farid, O.A. et al. (2021). Effects of Chronic Thermal Stress on Performance, Energy Metabolism, Antioxidant Activity, Brain Serotonin, and Blood Biochemical Indices of Broiler Chickens. Animals (Basel). 11(9):2554. doi: 10.3390/ani11092554.
  3. Saber, T.M. et al. (2021). Early postmortem biochemical, histological, and immunohistochemical alterations in skeletal muscles of rats exposed to boldenone undecylenate: Forensic implication. J Forensic Leg Med. 83:102248. doi: 10.1016/j.jflm.2021.102248.
  4. Tembo, M.C. et al. (2021). The Predictability of Frailty Associated with Musculoskeletal Deficits: A Longitudinal Study. Calcif Tissue Int. doi: 10.1007/s00223-021-00865-w.
  5. Delos Reyes, J.B. et al. (2021). Effects of dietary supplementation of vitamin C on productive performance, egg quality, tibia characteristics and antioxidant status of laying hens. Livest Sci. doi: 10.1016/j.livsci.2021.104502.
  6. Tembo, M.C. et al. (2021). Association between serum interleukin-6 and frailty in older men: cross-sectional data. Eur Geriatr Med. doi: 10.1007/s41999-021-00490-8.
  7. Abo El Gheit, R.E. et al. (2021). Retinoprotective effect of agmatine in streptozotocin-induced diabetic rat model: avenues for vascular and neuronal protection : Agmatine in diabetic retinopathy. J Physiol Biochem. doi: 10.1007/s13105-021-00799-9.
  8. Serrya, M.S. et al. (2021). Evaluation of the therapeutic effects of mycophenolate mofetil targeting Nrf-2 and NLRP3 inflammasome in acetic acid induced ulcerative colitis in rats. Life Sci. doi: 10.1016/j.lfs.2021.119154.
  9. Akgül, B. et al. (2021). Alleviation of prilocaine-induced epileptiform activity and cardiotoxicity by thymoquinone. Daru. doi: 10.1007/s40199-020-00385-2.
  10. Yu, D.G. et al. (2021). Effects of stocking density and dietary vitamin C on performance, meat quality, intestinal permeability, and stress indicators in broiler chickens. J Anim Sci Technol. doi: 10.5187/jast.2021.e77.
  11. Gross, J.J. et al. (2021). Metabolic and endocrine responses to short-term nutrient imbalances in the feed ration of mid-lactation dairy cows. Animal. 15(7):100306. doi: 10.1016/j.animal.2021.100306.
  12. Keller, M. et al. (2021). Antioxidant and Inflammatory Gene Expression Profiles of Bovine Peripheral Blood Mononuclear Cells in Response to Arthrospira platensis before and after LPS Challenge. Antioxidants (Basel). 10(5):814. doi: 10.3390/antiox10050814.
  13. El-Sadek, H.M. et al. (2021). Pentoxifylline treatment alleviates kidney ischemia/reperfusion injury: Novel involvement of galectin-3 and ASK-1/JNK & ERK1/2/NF-κB/HMGB-1 trajectories. J Pharmacol Sci. doi: 10.1016/j.jphs.2021.03.011.
  14. Bakeer, M.R. et al. (2021). Effect of dietary pumpkin (Cucurbita moschata) seed oil supplementation on reproductive performance and serum antioxidant capacity in male and nulliparous female V-Line rabbits. Ital. J. Anim. Sci. 20(1):419-425. doi: 10.1080/1828051X.2021.1889406.
  15. Lewandowski, L. et al. (2021). Concentration/activity of superoxide dismutase isozymes and the pro-/antioxidative status, in context of type 2 diabetes and selected single nucleotide polymorphisms (genes: INS, SOD1, SOD2, SOD3) – Preliminary findings. Biomed Pharmacother. doi:
  16. de Los Santos-Jiménez, J. et al. (2021). Glutaminase isoforms expression switches microRNA levels and oxidative status in glioblastoma cells. J Biomed Sci. 28(1):14. doi: 10.1186/s12929-021-00712-y.
  17. Ra, K. et al. (2021). Comparison of Anti-Oxidative Effect of Human Adipose- and Amniotic Membrane-Derived Mesenchymal Stem Cell Conditioned Medium on Mouse Preimplantation Embryo Development. Antioxidants (Basel). 10(2):268. doi: 10.3390/antiox10020268.
  18. Satpathy, S. et al. (2021). A fraction of Pueraria tuberosa extract, rich in antioxidant compounds, alleviates ovariectomized-induced osteoporosis in rats and inhibits growth of breast and ovarian cancer cells. PLoS One. 16(1):e0240068. doi: 10.1371/journal.pone.0240068.
  19. Gerszi, D. et al. (2021). Evaluation of oxidative/nitrative stress and uterine artery pulsatility index in early pregnancy. Physiol Int. doi: 10.1556/2060.2020.00041.
  20. Tsvetkova, A.S. et al. (2020). Melatonin Prevents Early but Not Delayed Ventricular Fibrillation in the Experimental Porcine Model of Acute Ischemia. Int J Mol Sci. 22(1):E328. doi: 10.3390/ijms22010328.
  21. Rondanelli, M. et al. (2020). Short- and Long-Term Effectiveness of Supplementation with Non-Animal Chondroitin Sulphate on Inflammation, Oxidative Stress and Functional Status in Obese Subjects with Moderate Knee Osteoarthritis before and after Physical Stress: A Randomized, Double-Blind, Placebo-Controlled Trial. Antioxidants (Basel). 9(12):1241. doi: 10.3390/antiox9121241.
  22. Domingo, J.C. et al. (2020). Are Circulating FGF21 and NT-proBNP promising novel biomarkers in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome? Antioxid Redox Signal. doi: 10.1089/ars.2020.8230.
  23. Manzocchi, E. et al. (2020). Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception. J Dairy Sci. doi: 10.3168/jds.2020-18602.
  24. Silva-Guillen, Y. et al. (2020). Growth performance, oxidative stress, and antioxidant capacity of newly weaned piglets fed dietary peroxidized lipids with vitamin E or phytogenic compounds in drinking water. Applied Animal Science. doi: 10.15232/aas.2019-01976.
  25. Drobnic, F. et al. (2020). Efficient Muscle Distribution Reflects the Positive Influence of Coenzyme Q10 Phytosome in Healthy Aging Athletes after Stressing Exercise. J Food Sci Nutr Res. 3(4):262-275. doi: 10.26502/jfsnr.2642-11000054.
  26. Moustafa, A. (2020). Effect of Light-Dark Cycle Misalignment on the Hypothalamic-Pituitary-Gonadal Axis, Testicular Oxidative Stress, and Expression of Clock Genes in Adult Male Rats. Int J Endocrinol. doi: 10.1155/2020/1426846.
  27. Sen, U. et al. (2020). Differential sensitivities of triple-negative breast cancer stem cell towards various doses of vitamin C: An insight into the internal antioxidant systems. J Cell Biochem. doi: 10.1002/jcb.29863.
  28. Guedj, F. et al. (2020). Apigenin as a Candidate Prenatal Treatment for Trisomy 21: Effects in Human Amniocytes and the Ts1Cje Mouse Model. Am J Hum Genet. doi: 10.1016/j.ajhg.2020.10.001.
  29. Moustafa, A. et al. (2020). Changes in nitric oxide, carbon monoxide, hydrogen sulfide and male reproductive hormones in response to chronic restraint stress in rats. Free Radic Biol Med. doi: 10.1016/j.freeradbiomed.2020.10.315.
  30. Palani, A. et al (2020). Impact of oxidative stress on semen parameters in normozoospermic infertile men: a case–control study. Afr J Urol. doi: 10.1186/s12301-020-00061-6.