Superoxide Dismutase (SOD) Activity Assay

Superoxide Dismutase (SOD) Activity Assay
  • Measure superoxide dismutase activity using a standard microplate reader
  • Suitable for use with plasma, serum, cells or tissues
  • Superoxide dismutase standard included for absolute quantitation


Frequently Asked Questions about this product

General FAQs about Oxidative Stress

Email To BuyerPrint this PageCopy Link

Please contact your distributor for pricing.

OxiSelect™ Superoxide Dismutase Activity Assay, Trial Size
Catalog Number
20 assays
Manual/Data Sheet Download
SDS Download
OxiSelect™ Superoxide Dismutase Activity Assay
Catalog Number
100 assays
Manual/Data Sheet Download
SDS Download
Product Details

Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD.

Our OxiSelect™ Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen.

Assay Principle for the OxiSelect™ Superoxide Dismutase Activity Assay.

Standard Curve Generated with the OxiSelect™ Superoxide Dismutase Activity Assay.

Recent Product Citations
  1. Wang, X. et al. (2020). Upregulation of microRNA-200a in bone marrow mesenchymal stem cells enhances the repair of spinal cord injury in rats by reducing oxidative stress and regulating Keap1/Nrf2 pathway. Artif Organs. doi: 10.1111/aor.13656.
  2. Dongil, P. et al. (2020). PAS kinase deficiency reduces aging effects in mice. Aging (Albany NY). doi: 10.18632/aging.102745.
  3. Xia, X. et al. (2020). Polyvinyl chloride microplastics induce growth inhibition and oxidative stress in Cyprinus carpio var. larvae. Sci Total Environ. doi: 10.1016/j.scitotenv.2019.136479.
  4. Cui, R. et al. (2019). Exendin-4 Protects Human Retinal Pigment Epithelial Cells from H2O2-Induced Oxidative Damage via Activation of NRF2 Signaling. Ophthalmic Res. doi: 10.1159/000504891.
  5. Nafiu, A.B. et al. (2019). Anti-androgenic and insulin-sensitizing actions of Nigella sativa oil improve polycystic ovary and associated dyslipidemia and redox disturbances. J Complement Med Res. 10(4): 186-199. doi: 10.5455/jcmr.20190613045154.
  6. Abdel-Moneim, A.E. et al. (2019). Effect of in Ovo Inoculation of Bifidobacterium spp. on Growth Performance, Thyroid Activity, Ileum Histomorphometry, and Microbial Enumeration of Broilers. Probiotics Antimicrob Proteins. doi: 10.1007/s12602-019-09613-x.
  7. Choi, J.S. et al. (2019). Evaluation of microplastic toxicity in accordance with different sizes and exposure times in the marine copepod Tigriopus japonicus. Mar Environ Res. doi: 10.1016/j.marenvres.2019.104838.
  8. Xu, A. et al. (2019). Protective effect of lycopene on testicular toxicity induced by Benzo[a]pyrene intake in rats. Toxicology. doi: 10.1016/j.tox.2019.152301.
  9. Karagenç, N. et al. (2019). Transfer of mouse blastocysts exposed to ambient oxygen levels can lead to impaired lung development and redox balance. Molecular Human Reproduction. doi: 10.1093/molehr/gaz052.
  10. Wang, S. et al. (2019). Effect of supplementation of pelleted hazel (Corylus avellana) leaves on blood antioxidant activity, cellular immune response and heart beat parameters in sheep. Journal of Animal Science. doi:10.1093/jas/skz288.
  11. Zhou, Z.B. et al. (2019). Up-regulation of heat shock protein 27 inhibits apoptosis in lumbosacral nerve root avulsion-induced neurons. Sci Rep. 9(1):11468. doi: 10.1038/s41598-019-48003-9.
  12. Abdul Salam, T.M. et al. (2019). Melatonin combination with perindopril alleviated doxorubicin cardiac toxicity in L-NAME hypertensive rats: comparative study with perindopril. PeerJ Preprints. 7:e27863v1. doi: 10.7287/peerj.preprints.27863v1.
  13. Lima, T. et al. (2019). Effect of Macleaya cordata and Magnolia officinalis plant extracts on oxidative stress control in lambs fed a high-concentrate diet. AJAS. doi: 10.5713/ajas.19.0050.
  14. Mehta, N. et al. (2019). Follistatin Protects against Glomerular Mesangial Cell Apoptosis and Oxidative Stress to Ameliorate Chronic Kidney Disease. Antioxid Redox Signal. doi: 10.1089/ars.2018.7684.
  15. Li, N. et al. (2019). Bilberry anthocyanin improve the serum cholesterol profile in aging perimenopausal rats via the estrogen receptor signaling pathway. Food & Function. doi:10.1039/c9fo00639g.
  16. Karthivashan, G. et al. (2019). Cognitive-enhancing and ameliorative effects of acanthoside B in a scopolamine-induced amnesic mouse model through regulation of oxidative/inflammatory/cholinergic systems and activation of the TrkB/CREB/BDNF pathway. Food Chem Toxicol. pii: S0278-6915(19)30261-3. doi: 10.1016/j.fct.2019.04.062.
  17. Lei, X.J. et al. (2019). Evaluation of supplementation of defatted black soldier fly (Hermetia illucens) larvae meal in beagle dogs. Annals of Animal Science. doi: 0.2478/aoas-2019-0021.
  18. Smith, O.S. et al. (2019). Plasma level of antioxidants is related to frequency of vaso-occlusive crises in children with sickle cell anaemia in steady state in nigeria. Pediatric Hematology Oncology Journal. doi: 10.1016/j.phoj.2019.03.003.
  19. Nishu, S.D. et al. (2019). Nutritional status regulates algicidal activity of Aeromonas sp. L23 against cyanobacteria and green algae. PLoS One. 14(3):e0213370. doi: 10.1371/journal.pone.0213370.
  20. Hong, E.Y. et al. (2019). Inhibitory Effects of Roseoside and Icariside E4 Isolated from a Natural Product Mixture (No-ap) on the Expression of Angiotensin II Receptor 1 and Oxidative Stress in Angiotensin II-Stimulated H9C2 Cells. Molecules. 24(3). pii: E414. doi: 10.3390/molecules24030414.
  21. Yao, Z. et al. (2019). BET inhibitor I-BET151 sensitizes GBM cells to temozolomide via PUMA induction. Cancer Gene Ther. doi: 10.1038/s41417-018-0068-4.
  22. Montero, N. et al. (2019). Biocompatibility studies of intravenously administered ionic-crosslinked chitosan-BSA nanoparticles as vehicles for antitumour drugs. Int J Pharm. 554:337-351. doi: 10.1016/j.ijpharm.2018.11.027.
  23. Chen, K.H. et al. (2019). Elevation of serum oxidative stress in patients with retina vein occlusions. Acta Ophthalmol. 97(2):e290-e295. doi: 10.1111/aos.13892.
  24. Dongil, P. et al. (2018). Pas Kinase Deficiency Triggers Antioxidant Mechanisms in the Liver. Sci Rep. 8(1):13810. doi: 10.1038/s41598-018-32192-w.
  25. Pool, H. et al. (2018). Development of genistein-PEGylated silica hybrid nanomaterials with enhanced antioxidant and antiproliferative properties on HT29 human colon cancer cells. Am J Transl Res. 10(8):2306-2323.
  26. Lee, H.W. et al. (2018). Cyanobacteria-specific algicidal mechanism of bioinspired naphthoquinone derivative, NQ 2-0. Sci Rep. 8(1):11595. doi: 10.1038/s41598-018-29976-5.
  27. Mascarenhas, S. et al. (2018). Silica - A trace geogenic element with emerging nephrotoxic potential. Sci Total Environ. 645:297-317. doi: 10.1016/j.scitotenv.2018.07.075.
  28. Alberdi, E. et al. (2018). Mangiferin and Morin Attenuate Oxidative Stress, Mitochondrial Dysfunction, and Neurocytotoxicity, Induced by Amyloid Beta Oligomers. Oxid Med Cell Longev. 2018:2856063. doi: 10.1155/2018/2856063.
  29. Li, L.F. et al. (2018). Attenuation of ventilation-induced diaphragm dysfunction through toll-like receptor 4 and nuclear factor-κB in a murine endotoxemia model. Lab Invest. 98(9):1170-1183. doi: 10.1038/s41374-018-0081-0.
  30. Liu, L. et al. (2018). The effect of noise exposure on insulin sensitivity in mice may be mediated by the JNK/IRS1 pathway. Environ Health Prev Med. 23(1):6. doi: 10.1186/s12199-018-0694-3.