Selection Guide for Oxidative Stress Assays by Sample Type

Selecting assays for oxidative stress begins with your samples. There are many markers of oxidative stress, but some are more easily detected in certain sample types (cells, tissues, urine, blood, etc.). We have developed a broad portfolio of sensitive, easy-to-use assays to quantify oxidative stress. Use the following table to determine the best assays for your sample type.

Marker or Type of Damage Sample Type
Cells Tissues Blood Urine Other
DNA / RNA Damage
8-hydroxyguanosine (8-OHG) X X X X  Cerebrospinal fluid
8-hydroxydeoxyguanosine (8-OHdG) X X X X  
Abasic (AP) sites X X      
BPDE DNA Adduct X X      
Double-strand DNA breaks X        
Comet Assay (general DNA damage) X        
UV DNA Damage (CPD, 6-4PP) X        
Lipid Peroxidation
4-Hydroxynonenal (4-HNE) X X X    
8-iso-Prostaglandin F2alpha (8-isoprostane) X X X X  
Malondialdehyde (MDA) X X X X  
TBARS X X X X  
Protein Oxidation / Nitration
Protein Carbonyl Content (PCC) X X X    
3-Nitrotyrosine X X X    
Advanced Glycation End Products (AGE) X X X    
Advanced Oxidation Protein Products (AOPP) X X X    
BPDE Protein Adduct X X X    
Kidney Injury Molecule-1 (KIM-1)       X  
Reactive Oxygen Species
Universal ROS / RNS X X X X  
Hydrogen Peroxide / Peroxidase X X X X  
Nitric Oxide X        
Antioxidants
Catalase X X X    
Glutathione X X X X  
Superoxide Dismutase X X X    
Oxygen Radical Antioxidant Capacity (ORAC) X X X X  Food samples
Hydroxyl Radical Antioxidant Capacity (HORAC) X X X X  Food samples
Total Antioxidant Capacity (TAC) X X X X  Food samples
Cellular Antioxidant Assay          Food samples, Antioxidants

 

View All Oxidative Stress Assays