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Q: Can this kit be used with samples from any species?
A: Yes, our Catalase Assays are not species specific and should work with any biological protein sample because they measure enzymatic activity rather than protein structure.
Q: Is it possible to test samples that have been stored…
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Q: What is the assay principle?
A: The catalase present in the sample breaks down hydrogen peroxide into water and oxygen. The remaining hydrogen peroxide then reacts with ADHP, which produces fluorescence that is measured as an assay read out. This results in a reverse curve, where less…
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Q: Can this kit be used with samples from any species?
A: Yes, our Total Glutathione Assay is not species specific and should work with any biological protein sample because it measures enzymatic activity rather than protein structure.
Q: Is it possible to test samples that have been stored…
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Q: Can this kit be used with samples from any species?
A: Yes, our SOD Activity Assay is not species specific and should work with any biological protein sample, because it measures enzymatic activity using a xanthine/xanthine oxidase method and does not depend on protein structure which can…
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Q: How does this assay work?
A: Our Total Antioxidant Capacity Assay is a colorimetric assay that quantifies antioxidant activity via a SET mechanism and is based on the ability of an antioxidant to reduce copper(II) ions to copper(I). The assay limit of detection is 0.0039 mM uric acid.
Q: Can…
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Q: Can you please explain the difference between the ORAC, HORAC, and TAC assays and why I would select one over the other?
All three assays measure antioxidant capacity but are slightly different. The ORAC and HORAC assays are both fluorometric assays that use HAT chemistry to detect…
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Q: Can you please explain the difference between the ORAC, HORAC, and TAC assays and why I would select one over the other?
A: All three assays measure antioxidant capacity but are slightly different. The ORAC and HORAC assays are both fluorometric assays that use HAT chemistry to detect…
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Q: What are the differences between the Intracellular ROS Assay and the In Vitro ROS Assay?
A: The main difference between these assays is the sample that is used. Our Intracellular ROS Assay is performed on cultured cells. The cell permeable DCFH-DA is added to cells and is hydrolyzed by…
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Q: What are the differences between the Intracellular ROS Assay and the In Vitro ROS Assay?
A: The main difference between these assays is the sample that is used. Our Intracellular ROS Assay is performed on cultured cells. The cell permeable DCFH-DA is added to cells and is hydrolyzed by…
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Q: Can these kits be used on frozen samples?
A: Both assays #STA-844 and #STA-344 can be used on frozen samples, but due to the transient nature of ROS samples should be frozen for no longer than 1-2 months at -80ºC. The best results will come from fresh samples.
Q: Are cell and tissue samples…
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Q: How do I determine which viral vector to use for gene delivery?
A: There are several considerations when deciding on the best viral vector:
Do I need transient or stable gene expression?
Do I need to infect dividing or non-dividing cells?
How important is potential immune response…
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Q: How should these cells be cultured?
A: The 293AAV cell line was derived from parental HEK293 cells, and the cells were specifically selected to have flattened morphology and firm attachment to improve viral applications. These cells can be cultured the same way you would culture parental 293…
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Q: What are the advantages to using AAV for gene delivery?
A: There are three main advantages to using AAV:
AAV has not been reported to cause any diseases. Together with its replication defective nature, AAV has a good safety profile to be used in gene transfer in vivo and as potential gene…
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Q: What is a bicistronic expression system?
A: A bicistronic vector contains an IRES element that controls the translation of a downstream gene, such as GFP. This means that translation of the GFP mRNA is independent of translation of the gene of interest that is cloned into the MCS, which is…
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Q: Is your ViraDuctin™ AAV Transduction Kit compatible with all cell types?
A: Our ViraDuctin™ AAV Transduction kit works by affecting DNA synthesis, the rate limiting step of viral transduction, and is not cell type or AAV serotype specific. We have not tested it on all cell types; it should…
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Q: Will your AAV Purification kits work with any AAV serotype?
A: Our AAV purification kits use an affinity matrix to purify AAV-2 based on the structure of the AAV2 Cap protein; therefore, they only work for AAV-2 and AAV-DJ and are not suitable for purification of other AAV serotypes. Other…
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Q: Will the QuickTiter™ AAV Quantitation Kit work on all AAV serotypes?
A: This kit can be used on purified AAV of any serotype, including AAV samples that have undergone ultracentrifugation procedures. For crude, unpurified AAV it will only work on AAV serotype 2 and AAV-DJ.
Q:…
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Q: How are these cells improved over normal 293 cells?
A: The HEK293 cell line is a permanent line established from primary embryonic human kidney transformed with human adenovirus E1 gene. 293AD is derived from the parental 293 cell line, but was specifically selected to have flattened…
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Q: What are the main differences between your two Adenovirus Purification Kits?
A: There are a couple of differences:
1. #VPK-099 uses a spin column while #VPK-5112 uses a purification resin.
2. #VPK-099 can purify one T75 flask or one 10cm dish per prep, while #VPK-5112 can handle up to…
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Q: Your ViraBind™ Adenovirus Purification Kit is listed as a 10 prep kit. Why does the kit only contain 5 filters?
A: The syringe-type filters found in this kit are designed to be used twice. Please see the product manual for the protocol to regenerate the filter for the second use, which will…
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Q: Which serotypes are your premade adenoviruses?
A: All of our Premade Recombinant Adenoviruses contain the Ad5 backbone with E1 and E3 deletions.
Q: What is the promoter for your premade adenoviruses?
A: All of our Premade Recombinant Adenoviruses use the CMV promoter.
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Q: How much adenovirus should be used for transduction?
A: The volume of supernatant required to infect cells will be different for every cell type and will be dependent on the MOI (multiplicity of infection) for that cell type. We recommend determining the MOI either by performing a…
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Q: What is the RAPAd® system?
A: The RAPAd® Adenoviral Expression System was developed by Dr. Beverly Davidson of University of Iowa. It uses an easy protocol that generates high titer virus in 2-3 weeks. Each RAPAd system contains a shuttle vector, a pacAD5 9.2-100 vector, a GFP control…
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Q: What cells can be infected with adenovirus?
A: Adenovirus is able to infect any cell type that expresses the CAR receptor, which includes most cell types. A literature search can be performed to determine if this receptor is expressed in a specific cell line or if other researchers have had…
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Q: Is there a positive control cell line that is easily infected by lentivirus?
A: We don’t have a control cell line, but we suggest using an easy to infect cell line such as 293T or our own 293LTV cell line. For ecotropic lentivirus we recommend NIH3T3 cells.
Q: How does your ViraDuctin™…